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RS ISO 9308-1: 2014
Water quality — Enumeration of Escherichia coli and coliform bacteria — Part 1: Membrane filtration method for waters with low bacterial background flora

Abstract

This part of ISO 9308 specifies a method for the enumeration of Escherichia coli (E. coli) and coliform
bacteria. The method is based on membrane filtration, subsequent culture on a chromogenic coliform
agar medium, and calculation of the number of target organisms in the sample. Due to the low selectivity
of the differential agar medium, background growth can interfere with the reliable enumeration of
E. coli and coliform bacteria, for example, in surface waters or shallow well waters. This method is not
suitable for these types of water.
This part of ISO 9308 is especially suitable for waters with low bacterial numbers that will cause less
than 100 total colonies on chromogenic coliform agar (CCA). These may be drinking water, disinfected
pool water, or finished water from drinking water treatment plants.
Some strains of E. coli which are ?-D-glucuronidase negative, such as Escherichia coli O157, will not be
detected as E. coli. As they are ?-D-galactosidase positive, they will appear as coliform bacteria on this
chromogenic agar.

General Information

Code	Classification	Theme	Pages	Publisher	Availability
RS ISO 9308-1: 2014	ADOPTED ISO STANDARDS	Natural and applied sciences	23	First Edition Nov 2014	Available