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RS ISO 12966-4: 2015
Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 4: Determination by capillary gas chromatography

Abstract

This part of ISO12966 specifies a method for the determination of fatty acid methylesters(FAMEs) derived
by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography
(GLC). Fatty acid methyl esters from C8 to C24 can be separated using this part of ISO 12966 including
saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and
trans-polyunsaturated fatty acid methyl esters.
The method is applicable to crude, refined, partially hydrogenated, or fully hydrogenated fats, oils, and
fatty acids derived from animal and vegetable sources.
This method is not suitable for the analysis of dairy, ruminant fats and oils, or products supplemented
with conjugated linoleic acid (CLA). Milk and milk products (or fat coming from milk and milk products)
are excluded from the scope of this part of ISO 12966.
This part of ISO 12966 is not applicable to di-, tri-, polymerized and oxidized fatty acids, and fats and oils.

General Information

Code	Classification	Theme	Pages	Publisher	Availability
RS ISO 12966-4: 2015	ADOPTED ISO STANDARDS	Food technology	27	First Edition Sep 2023	Available